The temperature characteristics of emission-cross section spectra of Nd∶GdVO4 and Nd∶YVO4 crystals are studied experimentally. According to the relationship between the emission cross section spectra and the laser wavelength, the influence of temperature on the output spectra of Nd∶GdVO4 and Nd∶YVO4 dual-frequency microchip laser wavelengths is further investigated. The experimental results show that, in the studied temperature range, the central wavelength of the emission-cross section spectra of Nd∶GdVO4 and Nd∶YVO4 crystals shows red-shift and the peak decline. Both of them are linearly related to the temperature. The wavelength of the corresponding dual-frequency microchip laser also shows a linear red shift with temperature increasing, but the double frequency difference remains unchanged. The power balance of the dual-frequency laser changes with the common red-shift of the crystal emission cross section spectrum and the laser wavelength.