The new construction scheme of two dimensional optical orthogonal codes (2D-OOCs), of which auto-correlation side lobes are 0 and the cross-correlation side lobes are at most 1, and the interference estimate receiver of asynchronous frequency-hopping time-spreading two-dimensional optical code division multiple access (2D-OCDMA) system are presented. For constructing the 2D-OOCs, two unipolar codes are used as the frequency-hopping pattern and the time-spreading pattern, respectively. The unipolar codes used as frequency-hopping patterns are OOCs of which auto-correlation side lobes and the cross-correlation one are at most 1, for the unipolar codes used as the time-spreading patterns, the restriction is that their code lengths are a prime number or their code length and displacement difference between any two non-zero chips in the same code are co-prime number. By using one 2D-OOC as multi-user interference (MUI) estimation code, which is not assigned to any user, the asynchronous MUI estimate can be realized. According to the estimating of MUI, the optimum decision threshold can be obtained. The analysis of bit error rate (BER) indicates that the system with interference estimate receiver has advantage over traditional system.