Effects of different light intensity (0, 500, 2 000, 3 000 and 4 000 Lux) and light quality (blue, green and red light) on propagation of axillary buds by Clematis‘nelly moser’ are studied. MS media with NAA (Naphthaleneacetic Acid) 0.2 mg/L+6-BA(6-Benzylaminopurine) 2 mg/L are used as basal media. The experimental results show that the 3 000 Lux white light is better for the axillary buds propagation than the other light quality values. Under 3 000 Lux white light, the adventitious buds per explants, average adventitious bud height per explants and average nodes number are 100%, 4.4, 5.66 cm and 4.6, respectively. Besides, it is found that the blue light significantly enhanced the induction efficiency of adventitious buds, which is 1.27 times than that under white light treatments. The physiological and biochemical data show that the activities of superoxide dismutase (SOD), hydrogen peroxide (CAT), and the ratio of chlorophyll a to b in adventitious bud under blue light treatment are significantly higher than those in the other light quality treatments.