Effects of γ-ray irradiation and particle size on enzymatic hydrolysis and fermentation of reed straws

Hui QI; Liang CHEN; Xiaofen WU; An LIU; Danyang WANG; Yong ZHANG; Ming DENG; Keqin WANG

doi:10.11889/j.1000-3436.2022-0057

Journals >Journal of Radiation Research and Radiation Processing >Volume 41 >Issue 1 >Page 010402 > Article

Journal of Radiation Research and Radiation Processing
Vol. 41, Issue 1, 010402 (2023)

Effects of γ-ray irradiation and particle size on enzymatic hydrolysis and fermentation of reed straws

Hui QI¹, Liang CHEN¹, Xiaofen WU¹, An LIU¹, Danyang WANG², Yong ZHANG¹, Ming DENG¹, and Keqin WANG^1、*

Author Affiliations

¹Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences/ Hunan province Engineering Technology Research Center of Agricultural Biological Irradiation, Changsha 410125, China

²Longping Branch, Graduate School of Hunan University, Changsha 410125, China

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DOI: 10.11889/j.1000-3436.2022-0057 Cite this Article

Hui QI, Liang CHEN, Xiaofen WU, An LIU, Danyang WANG, Yong ZHANG, Ming DENG, Keqin WANG. Effects of γ-ray irradiation and particle size on enzymatic hydrolysis and fermentation of reed straws[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(1): 010402 Copy Citation Text

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Particle size distribution of crushed reed straws at different absorbed irradiation doses; a, b, c, d, there are significant differences in the same series with different letters (p<0.05)

Fig. 1. Particle size distribution of crushed reed straws at different absorbed irradiation doses; a, b, c, d, there are significant differences in the same series with different letters (p<0.05)

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Comminution energy of reed straws to different particle size ranges under different absorbed doses; a, b, c, d, there are significant differences in the same series with different letters (p<0.05)

Fig. 2. Comminution energy of reed straws to different particle size ranges under different absorbed doses; a, b, c, d, there are significant differences in the same series with different letters (p<0.05)

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Fig. 3. Cellulose enzymatic hydrolysis conversion rate of reed straws with different particle sizes under different absorbed doses; a, b, c, d, there are significant differences in the same series with different letters (p<0.05)

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Fig. 4. The cellulose ethanol conversion rates of reed straws with different particle sizes treated with different absorbed doses; a, b, c, d, there are significant differences in the same series with different letters (p<0.05)

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剂量 / kGy Dose	粒径r / mm Particle size	水提液中糖含量 / (mg·g^-1) Sugar content in water extract		可溶性聚糖含量 / (mg·g^-1) Soluble glycan content		纤维素含量 / % Cellulose content	半纤维素含量 / % Hemicellulose content	木质素含 / % Lignin content
剂量 / kGy Dose	粒径r / mm Particle size	葡萄糖 Glucose	木糖 Xylose	葡聚糖 Glucan	木聚糖 Xylan	纤维素含量 / % Cellulose content	半纤维素含量 / % Hemicellulose content	木质素含 / % Lignin content
0	All	0.049±0.005^g	0.183±0.002^h	0.815±0.044^j	1.211±0.010^m	39.546±0.372^a	18.827±0.000^b	22.708±0.808^b
	r˂0.850	0.101±0.011^g	0.380±0.005^g	1.101±0.022ⁱ	1.738±0.049^l	39.119±0.084^b	18.829±0.023^b	23.008±0.651^b
	r˂0.425	0.205±0.023^f	0.525±0.030^f	1.488±0.110^h	2.071±0.032^k	37.980±0.123^cde	18.858±0.075^b	23.500±0.332^ab
	r˂0.250	0.637±0.070^d	0.733±0.099^d	2.369±0.147^g	2.591±0.029^j	37.620±0.292^e	19.130±0.136^a	23.588±0.453^ab
	r˂0.180	1.089±0.120^b	0.845±0.088^bc	3.136±0.250^f	2.888±0.007ⁱ	36.669±0.255^g	19.181±0.140^a	23.648±0.611^ab
206	All	0.485±0.003^e	0.576±0.001^f	3.164±0.031^f	5.016±0.042^h	38.116±0.083^cd	18.114±0.033^d	23.560±0.285^ab
	r˂0.850	0.504±0.012^e	0.568±0.023^f	3.200±0.019^f	5.067±0.024^h	38.241±0.074^c	18.061±0.032^d	23.570±0.201^ab
	r˂0.425	0.617±0.007^d	0.610±0.023e^f	3.420±0.011^e	5.307±0.008^g	37.837±0.009^de	18.056±0.001^d	23.813±0.135^ab
	r˂0.250	0.936±0.036^c	0.705±0.046^de	3.971±0.008^d	5.843±0.001^f	37.008±0.175^fg	18.152±0.063^d	23.855±0.432^ab
	r˂0.180	1.258±0.047^a	0.774±0.047^cd	4.555±0.015^b	6.321±0.034^e	36.248±0.259^h	18.325±0.071^c	24.381±0.261^a
404	All	0.213±0.018^f	0.854±0.051^bc	4.087±0.027^cd	11.688±0.163^d	37.208±0.029^f	17.616±0.031^fg	23.293±0.099^ab
	r˂0.850	0.212±0.018^f	0.853±0.054^bc	4.090±0.027^cd	11.715±0.163^d	37.199±0.028^f	17.608±0.031^fg	23.287±0.105^ab
	r˂0.425	0.217±0.019^f	0.878±0.060^b	4.181±0.032^c	11.998±0.157^c	37.062±0.037^f	17.572±0.040^g	23.427±0.330^ab
	r˂0.250	0.262±0.016^f	0.989±0.022^a	4.644±0.038^b	12.992±0.075^b	36.013±0.133^h	17.752±0.055^ef	23.286±0.939^ab
	r˂0.180	0.277±0.023^f	1.060±0.022^a	5.153±0.018^a	13.837±0.031^a	35.147±0.182ⁱ	17.865±0.147^e	23.097±1.102^b

Table 1. Determination of components and components of water extract of irradiated reed straws under different particle sizes

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方差来源 Sources of variance	平方和 Sum of squares	自由度 Degree of freedom	均方和 Mean sum of square	F值 F value	显著性 Significance level
因素A（吸收剂量） Factor A (irradiation dose)	343.58	2	171.79	1 321.46	**
因素B（粒径大小） Factor B (particle size)	289.45	4	72.36	556.62	**
A×B	34.61	8	4.33	33.31	**
误差E Error E	3.90	30	0.13	-	-
总和 Sum	671.54	44	-	-	-

Table 2. Variance analysis of cellulose enzymatic hydrolysis conversion of reed straws with different absorbed doses and particle size

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方差来源 Sources of variance	平方和 Sum of squares	自由度 Degree of freedom	均方和 Mean sum of square	F值 F value	显著性 Significance level
因素A（吸收剂量） Factor A (absorbed dose)	46.03	2	23.02	958.98	**
因素B（粒径尺寸） Factor B (particle size)	46.81	4	11.70	487.63	**
A×B	10.12	8	1.27	52.73	**
误差E Error E	0.36	15	0.02	-	-
总和 Sum	103.33	29	-	-	-

Table 3. Variance analysis of cellulose ethanol conversion of reed straws with different absorbed doses and particle size

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粒径 / mm Particle size	粉碎能耗增加百分比 / % Increased ratios of comminution energy			纤维素酶解转化率增加百分比 / % Increased ratios of cellulose enzymatic hydrolysis conversion rates			纤维素乙醇转化率增加百分比 / % Increased ratios of cellulose ethanol conversion rates
粒径 / mm Particle size	0 kGy	206 kGy	404 kGy	0 kGy	206 kGy	404 kGy	0 kGy	206 kGy	404 kGy
r＜0.850	106.98	8.55	1.73	108.55	57.45	42.18	93.88	75.70	12.73
r＜0.425	318.08	35.48	18.75	135.96	63.47	47.89	116.58	75.15	13.12
r＜0.250	1 200.60	216.88	95.65	153.90	61.62	82.46	128.73	75.95	14.89
r＜0.180	2 120.56	472.69	185.82	129.20	85.98	106.63	136.04	80.20	17.68

Table 4. Increased ratios of comminution energy, cellulose enzymatic hydrolysis conversion rates and cellulose ethanol conversion rates of reed straws passing through sieves with different particle sizes

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