A digital dispensing technology by using a pulse inertial force drived micro dispensing system without a heat resource and a pneumatic actuator for microfluids was presented to slove the existing problems on the huge external driving device,complicated configuration manufactured by MEMS technology and poor operability and compatibility with biological samples.The principle of digital dispensing technology of microfluids was introduced.Then,effects of the sample uniformity and driven parameters on dispensing technology were researched and the experimental data were analysed.Finally, the medium-density microarray was accomplished with the low viscosity liquid at 4.13 mPa·s.The experimental results show that the average diameter of spots is below 100 μm,and the Coefficients of Variation (CV) for diameters of spots are clearly below the measurement error (8%).It can satisfy the microarray requirements for the density,uniformity and sizes of spots,and can be used in small laboratories.