A 1024 linear CMOS image sensor (CIS) with programmable resolution and high speed was presented and verified. In order to optimize the column bus readout rate, this sensor adopted column bus segmentation technology, which effectively reduced the parasitic capacitance on the bus and increased the signal readout rate. The sensor featured 4 kind of programmable resolution for higher frame rate. In this paper, the chip was successfully manufactured by 0.5μm CMOS process, and the photoelectric response of the device was tested and evaluated. The results show that the sensors full well charge is 4.76Me-/pixel, and dynamic range is 75dB. At 128 resolution, frame rate reaches 36000frames/s.