Tunable Diode Laser Absorption Spectroscopy (TDLAS) technology and Computed Tomography (CT) technology were combined to reconstruct two-dimensional temperature distribution of bunsen burner flame through getting the most fitting spectrum. Zeromean Normalization Cross Correlation (ZNCC) value and Sum of Squared Difference (SSD) value were used as target to analyze accuracy of 32-path reconstruct temperature distribution. And ZNCC value comes to 0.994, SSD value comes to 0.000 86. 32-path CT-TDLAS system was designed and applied to measure the two-dimensional temperature distribution of Bunsen burner flame, and compared with the results of thermocouple. The results show that the temperature distribution trends between thermocouple and TDLAS are same while CT-TDLAS has higher precision and resolution. The feasibility and accuracy of reconstruction using tunable diode laser absorption spectroscopy technology and computed tomography technology is demonstrated.