The auto location control to spectral scanning of sample detection is one of the key technologies of fast biochemical detectors with multi-parameter. According to fast micro-biochemical detectors with multi-parameter researched by Chongqing University based on micro-spectrometer, the design of auto-location control systems for multi-parameter sample detection spectral scanning is referred and finished. And an online debugging experiment research on multi-parameter fast micro-biochemical detectors based on the control system is developed. Experimental results show that the variation coefficient of sample detection absorbance CV is equal and less than 1%, the linear coefficient of absorbance R2 is equal and more than 0.995. And the relative technology requirements of semiautomatic biochemical detector are met.