Corneal contour measurement has significant meaning in ophthalmology, but it is difficult because of high transmittance of cornea. According to the characteristics of cornea and by taking advantage of non-diffracting grating structure light projection system, the contour of corneal surface coated with fluorescein is measured. A model of non-diffracting grating structure light projection system is proposed and experiment of corneal surface contour measurement is designed. A measurement platform is set to realize rabbit corneal contour measurement, and corneal deforming phenomenon caused by increasing intraocular pressure is observed. Experimental result shows that the corneal contour can be measured precisely by non-diffracting grating projection system. Feasibility of intraocular pressure measurement according to variation of corneal contour curvature is validated.