A polarizing measurement system is designed which could acquire Mueller matrix of the target cells. The system utilizes a liquid crystal modulator bonding with polarization splitter prism as its key component, to realize Mueller matrix measurement with a period of 10-time light intensity. This method can easily acquire real-time data divided from Mueller matrix, which is more efficient than other method. Besides the specific design and the experiment result are presented based on this system. The analysis and discussion of measurement error are also included, which indicates that this system’s error can be controlled within 2%.