Ecological consolidation is of great importance for land consolidation, and it is also the inevitable requirement of ecological civilization construction and rural revitalization strategy. The spatial zoning of ecological consolidation and differentiated management can contribute to economic development and human well-being. In this study, we aimed to characterize the relationship between ecosystem services supply and demand, and identify their spatial heterogeneity with Guangxi as a case study. Firstly, the ecosystem services supply was calculated by using revised value method, and the ecosystem services demands were characterized by using the socio-economic indicators. Secondly, through conducting the relationship framework between land ecological consolidation and ecosystem services, we analyzed and evaluated ecosystem service supply-demand space matching status, relationship, aggregation and coordination degree based on matching types, local spatial autocorrelation analysis and coordination degree. Finally, the spatial zoning of land ecological consolidation, and its regulation measures in different subareas were determined and discussed. The results showed that: (1) Both ecosystem service supply and demand of Guangxi had high spatial variations. The high supply of ES was mainly distributed in the mountainous forest areas or ecological function districts around Guangxi, while the low supply of ES was concentrated in cities. Ecosystem service demands were high in the southeastern Guangxi, whereas they were low in the northwestern Guangxi. (2) The differences of ecosystem services supply and demand spatial matching were obvious, and were grouped into four matching types. The average coordination degree of ecosystem services was 0.531, which was in a general equilibrium level, indicating that ecosystem services supply and demand was in an equilibrium state. (3) Based on the combination of the geographical environment characteristics, landscape heterogeneity and ecological function zoning, the 94 administrative county units in Guangxi could be classified into four land ecological management areas (HH area, LH area, LL area, and HL area), and 10 land ecological subareas. Differential management measures were proposed for each management area. HH area should focus on conservation and cultivation, and prohibit the large-scale construction. HL area should be protected comprehensively to improve production efficiency and management level. LH area should improve land use efficiency and implement comprehensive improvement measures. LL area should target at ecological reconstruction.