Qiemo was an ancient country on the south branch of the Silk Road. The Zagunluke tomb site is located at the Qiemo County of the Xinjiang Uygur Autonomous Region. Glass beads and only colourless glass cup were excavated from the 3rd cultural layer of the tomb site M133 and M49, dated between the 1st AD-6th AD. LA-ICP-AES was applied to analyse chemical composition of these glass finds with the corning glass as reference. According to the result, characteristics of chemical composition are very similar to typical soda-lime glass, which indicates the glasses were imported productions from the west. These soda-lime glasses were divided into two groups in terms of flux source: natron glass and plant ash glass. This analytical research indicates the history of glass trade and communication between the East and the West on the Silk Road.