Ganoderma lucidum,ganoderma atrum,ganderma tsugae Murr. and ganoderma lipsiense can be discriminated and identified by using multi-steps infrared macro-fingerprint method. The 1D-IR spectra,based on the peaks intensity at 1 153 and 1 078 cm-1,which are the fingerprint characteristic peaks of glucoside compounds,show that the content of glucoside compounds of them was in the order of,ganoderma lucidum＞ganoderma atrum＞ganderma tsugae Murr. ＞ganoderma lipsiense. Generally,the second derivative IR spectra can clearly enhance the spectra resolution. In the range of 1 600-1 720 cm-1,the position and sharpness of characteristics peaks were very different,and it’s proved that amino acid peptide compounds of them were different. In the 2D-IR spectra,four of them have the same autopeak at 1 100 cm-1,which is the autopeaks of glucoside,but the number of autopeaks of ganoderma lucidum was 4 and its strongest autopeak was 1 040 cm-1,while 5 autopeaks,4 autopeaks and 5 autopeaks were for ganoderma atrum,ganderma tsugae Murr. and ganoderma lipsiense respectively,and their strongest autopeaks were 1 040,1 139,1 140 and 1 134 cm-1 respectively. The multi-steps infrared maro-fingerprint identification testified that the contents of glucoside compounds and amino acid peptide compounds in these four kinds of ganoderma are different. It’s proved that multi-steps infrared maro-fingerprint method can be used to analyze and distinguish ganoderma lucidum,ganoderma atrum,ganderma tsugae Murr. and ganoderma lipsiense.