Acid rain is a worldwide environmental problem. Serious acid rain pollution in subtropical China has constituted a potential threat to the health of the local forest. In the present paper,the changing properties of the chlorophyll concentration and spectral reflectance at the visible wavelengths for the six subtropical broad-leaved tree species’ leaves under simulated acid rain (SAR) treatment with different pH levels were studied. With the increasing strength of the SAR,the chlorophyll concentrations of the experimental species under pH 2.5 and pH 4.0 treatment were higher than that under pH 5.6;the spectral reflectance at the visible wavelengths for pH 2.5 and pH 4.0 were lower than that for pH 5.6 in general;while there weren’t significant differences between pH 2.5 and pH 4.0. After the treatment with different levels of SAR,the differences in spectral reflectance at the visible wavelengths mainly focused around the green peak and red edge on the reflectance curve. The subtropical broad-leaved tree species studied were relatively not sensitive to acid rain stresses;some stronger acid rain may accelerate the growth of the tree species used here to some extent.