A flow injection- hydride generation-atomic absorption spectrometricmethod for the determination of selenium in poultry eggs was developed. Varioustesting conditions and effect factors for the determination of selenium werestudied. The authors also discussed the content of selenium in poultry eggs andthe application of scientific feed method in improving selenium content inpoultry eggs. The detection limit for selenium was 0.25 μg?L-1 under optimumconditions. The linear range for selenium was 0.25-60 μg?L-1. The relativestandard deviation was less than 2.5% and the recovery was 95%-108%. This methodovercame the problem of severe matrix interferences of graphite furnace atomicabsorption spectrometry, in which matrix modifier must be added to eliminatematrix interference by raising ashing temperature. Additionally this methodovercame the shortcoming of slow analysis procedure, complex operation and errorcaused by manual injection in traditional interval hydride generation-atomicabsorption spectrometry. It is a simple, rapid, highly sensitive and automaticmethod and has been applied to the determination of selenium in poultry eggs withsatisfactory results.Poultry eggs; Selenium; Determination of content