Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components

Yalu QIU; Peng GAO; Jiang HE; Qian CHEN; Yan WANG

doi:10.11889/j.1000-3436.2023-0007

时间 / min Time

流速 /（mL∙min^-1）Velocity

乙腈 / % Acetonitrile

0.5%冰醋酸 / % Glacial acetic acid

0.00

1.00

10.0

90.0

15.00

1.00

10.0

90.0

30.00

1.00

20.0

80.0

35.00

1.00

30.0

70.0

40.00

1.00

55.0

45.0

45.00

1.00

55.0

45.0

55.00

1.00

95.0

5.0

60.00

1.00

95.0

5.0

65.00

1.00

10.0

90.0

70.00

1.00

10.0

90.0

序号Serial

Number

组分

Components

出峰时间 / min

Peak time

判定系数

Judgment coefficient

线性范围 /（μg∙mL^-1）

Linear range

绿原酸Chlorogenic acid

11.807~11.920

0.997 83

12.26~61.30

川芎嗪Ligustrazine

14.780~15.093

0.999 70

19.60~98.00

阿魏酸Ferulic acid

31.520~31.597

0.999 85

9.86~49.28

阿魏酸松柏酯Coniferly ferulate

45.520~45.647

0.999 75

10.00~50.00

洋川芎内酯Senkyunolide

48.820~48.857

0.999 60

100.00~500.00

正丁基苯酚N-butyl phenol

49.470~49.507

0.999 97

10.00~50.00

藁本内酯Ligustilide

51.950~51.967

0.999 99

100.00~500.00

欧当归内酯Levistilide

56.410~56.413

0.999 95

16.50~82.50

组分

Components

低浓度

/ (μg∙mL^-1)

Low concentration

实测浓度

/ (μg∙mL^-1)Measured concentration

回收率 / %

Rate of recovery

中浓度

/ (μg∙mL^-1)

Medium concentration

实测浓度

/ (μg∙mL^-1)Measured concentration

回收率 / %

Rate of recovery

高浓度

/ (μg∙mL^-1)

High concentration

实测浓度

/ (μg∙mL^-1)Measured concentration

回收率 / %

Rate of recovery

绿原酸Chlorogenic acid

18.36

17.12

93.25

147.12

134.48

91.41

588.48

539.63

91.70

17.56

95.64

140.70

95.64

564.45

95.92

18.41

100.32

144.13

97.97

547.32

93.01

川芎嗪Ligustrazine

29.40

27.34

92.99

235.20

217.68

92.55

940.80

854.88

90.87

28.70

97.62

225.91

96.05

910.12

96.74

26.65

90.65

227.13

96.57

890.45

94.65

阿魏酸Ferulic acid

14.76

14.30

96.72

118.26

108.43

91.69

473.04

452.76

95.71

13.63

92.19

110.86

93.74

430.33

90.97

14.31

96.79

114.08

96.47

447.34

94.57

阿魏酸松柏Coniferly ferulate

15.00

13.76

91.73

120.00

123.11

102.59

480.00

452.35

94.24

15.35

102.33

108.97

90.81

471.33

98.19

14.70

98.00

117.38

97.82

467.42

97.38

洋川芎内酯Senkyunolide

15.00

15.64

104.27

120.00

120.61

100.51

480.00

465.12

96.90

14.43

96.23

124.10

103.42

475.87

99.14

14.73

98.17

123.36

102.80

470.40

98.00

正丁基苯酚N-butyl phenol

15.00

14.95

99.67

120.00

119.37

99.48

480.00

498.81

103.92

15.11

100.73

118.80

99.00

481.50

100.31

14.64

97.60

117.52

97.93

486.17

101.29

藁本内酯Ligustilide

15.00

14.25

95.00

120.00

117.27

97.73

480.00

460.92

96.03

14.74

98.27

115.38

96.15

456.12

95.03

14.47

96.47

115.50

96.25

451.39

94.04

欧当归内酯Levistilide

24.75

22.75

91.92

198.00

183.39

92.62

792.00

769.23

97.13

23.22

93.82

181.40

91.62

780.69

98.57

23.51

94.99

179.18

90.49

754.86

95.31

组分

Components

第1次

First time

第2次

Second time

第3次

Third time

第4次Fourth time

第5次

Fifth time

第6次

Sixth time

平均值

Average value

RSD / %

绿原酸

Chlorogenic acid

0.544 3

0.530 2

0.512 1

0.513 4

0.512 5

0.514 2

0.521 1

2.55

川芎嗪

Ligustrazine

0.041 0

0.041 3

0.041 1

0.041 2

0.041 3

0.041 2

0.27

阿魏酸

Ferulic acid

1.015 3

1.095 2

1.078 5

1.016 1

1.173 3

1.167 6

1.091 0

0.64

阿魏酸松柏酯

Coniferly ferulate

0.031 2

0.031 3

0.031 2

0.031 3

0.031 2

0.07

洋川芎内酯

Senkyunolide

1.323 4

1.322 5

1.321 1

1.321 0

1.321 4

1.321 0

1.321 7

0.08

正丁基苯酚

N-butyl phenol

0.023 1

0.023 2

0.023 1

0.023 2

0.023 1

0.023 2

0.27

藁本内酯

Ligustilide

4.432 0

4.428 0

4.436 0

4.426 0

4.422 0

4.424 0

4.428 0

0.12

欧当归内酯

Levistilide

0.122 0

0.121 8

0.122 6

0.122 9

0.121 8

0.122 1

0.122 2

0.37

组分

Components

0 h

2 h

4 h

8 h

12 h

平均值

Average value

RSD / %

绿原酸

Chlorogenic acid

0.561 2

0.548 7

0.546 6

0.559 6

0.529 7

0.549 2

2.30

川芎嗪

Ligustrazine

0.043 3

0.046 4

0.045 3

0.043 8

0.044 6

0.044 7

2.75

阿魏酸

Ferulic acid

1.024 5

1.033 6

1.024 8

1.025 2

1.033 0

1.028 2

0.45

阿魏酸松柏酯

Coniferly ferulate

0.046 5

0.047 8

0.048 1

0.046 5

0.048 7

0.047 5

2.07

洋川芎内酯

Senkyunolide

1.350 2

1.329 7

1.353 6

1.348 6

1.332 7

1.343 0

0.81

正丁基苯酚

N-butyl phenol

0.037 4

0.035 3

0.038 2

0.036 9

0.037 0

2.87

藁本内酯

Ligustilide

4.431 0

4.428 0

4.431 0

4.410 7

4.428 2

4.425 8

0.19

欧当归内酯

Levistilide

0.143 2

0.145 8

0.138 0

0.140 1

0.140 8

0.141 6

2.12

组分

Components

平均值

Average value

RSD / %

绿原酸

Chlorogenic acid

0.521 5

0.511 8

0.546 8

0.529 1

0.523 8

0.530 2

0.527 2

2.21

川芎嗪

Ligustrazine

0.044 6

0.046 0

0.046 5

0.044 3

0.045 5

0.045 8

0.045 5

1.86

阿魏酸

Ferulic acid

0.993 2

1.028 4

1.036 5

0.995 3

1.008 3

1.011 4

1.012 2

1.72

阿魏酸松柏酯

Coniferly ferulate

0.034 6

0.034 7

0.034 3

0.034 8

0.035 2

0.035 1

0.034 8

0.92

洋川芎内酯

Senkyunolide

1.235 8

1.269 1

1.312 0

1.322 5

1.288 9

1.336 8

1.294 2

2.89

正丁基苯酚

N-butyl phenol

0.028 9

0.028 5

0.029 9

0.030 3

0.029 8

0.029 4

0.029 5

2.29

藁本内酯

Ligustilide

4.468 9

4.418 1

4.434 7

4.429 6

4.423 8

4.435 4

4.435 1

0.40

欧当归内酯

Levistilide

0.133 3

0.131 7

0.132 4

0.129 7

0.132 8

0.133 2

0.132 2

1.02

S10

S11

S12

S13

S14

S15

1.000

0.995

0.996

0.994

0.996

0.997

0.990

0.993

0.994

0.995

0.988

0.990

0.993

0.996

0.995

1.000

0.992

0.996

0.997

0.996

0.992

0.995

0.994

0.995

0.992

0.994

0.997

0.996

0.992

1.000

0.995

0.997

0.998

0.995

0.994

0.996

0.991

0.993

0.995

0.997

0.994

0.996

0.995

1.000

0.999

0.998

0.999

0.998

0.999

0.998

0.999

0.996

0.997

0.998

1.000

0.997

0.998

0.999

0.995

0.996

0.998

0.999

0.997

0.996

0.998

0.999

1.000

0.996

0.997

0.998

0.999

0.994

0.996

0.997

0.999

0.990

0.992

0.995

0.999

0.997

0.996

1.000

0.999

1.000

0.999

0.998

0.999

1.000

0.999

0.998

0.993

0.995

0.994

0.998

0.997

0.999

1.000

0.999

1.000

0.999

0.993

0.994

0.996

0.999

0.998

1.000

0.999

0.998

0.999

1.000

0.999

S10

0.994

0.996

0.998

0.999

1.000

0.998

0.999

1.000

0.999

S11

0.994

0.996

0.999

0.998

0.999

1.000

0.998

0.999

1.000

0.999

S12

0.995

0.996

0.998

0.999

0.998

0.999

1.000

0.996

0.998

0.999

S13

0.988

0.992

0.991

0.998

0.995

0.994

0.999

0.998

0.996

1.000

0.999

0.997

S14

0.990

0.992

0.993

0.998

0.996

1.000

0.999

0.998

0.999

1.000

0.998

S15

0.993

0.994

0.995

0.999

0.998

0.997

0.999

1.000

0.999

1.000

0.999

0.996

0.997

0.999

0.998

0.999

0.997

0.998

0.999

1.000

吸收剂量 / kGyAbsorbed dose

绿原酸Chlorogenic acid

川芎嗪Ligustrazine

阿魏酸Ferulic acid

阿魏酸松柏酯 Coniferly ferulate

洋川芎内酯Senkyunolide

正丁基苯酚 N-butyl phenol

藁本内酯Ligustilide

欧当归内酯Levistilide

4.21

0.10

1.82

2.21

14.47

0.24

27.86

0.23

4.07

0.09

1.67

2.02

13.30

0.22

25.65

0.21

3.96

0.09

1.62

2.06

13.08

0.21

25.17

0.21

平均含量±SD

Average content±SD

4.08±0.12

0.09±0.01

1.70±0.10

2.10±0.10

13.62±0.75

0.22±0.01

26.23±1.43

0.22±0.01

3.55

0.09

1.90

1.88

14.20

0.25

26.18

0.21

3.64

0.09

2.14

1.96

14.04

0.25

25.81

0.21

3.89

0.09

2.28

1.72

14.28

0.25

27.87

0.23

平均含量±SD

Average content±SD

3.69±0.18

0.09±0.00

2.10±0.19

1.85±0.12

14.17±012

0.25±0.00

26.62±1.10

0.22±0.01

4.86

0.14

1.82

2.09

14.11

0.26

27.52

0.21

3.69

0.09

1.91

1.90

14.22

0.26

25.92

0.26

4.13

0.13

1.97

1.85

14.06

0.29

27.34

0.28

平均含量±SD

Average content±SD

4.23±0.59

0.12±0.03

1.90±0.08

1.95±0.13

14.13±0.08

0.27±0.02

26.93±0.88

0.25±0.04

2.17

0.05

1.11

1.93

7.84

0.15

15.37

0.16

3.53

0.08

1.73

1.60

12.89

0.26

24.62

0.25

3.37

0.09

1.90

1.23

12.54

0.25

23.59

0.24

平均含量±SD

Average content±SD

3.03±0.74

0.07±0.02

1.58±0.41

1.59±0.35

11.09±2.82

0.22±0.06

21.19±5.07

0.22±0.05

3.74

0.09

1.63

2.60

14.37

0.29

26.44

0.27

3.87

0.12

1.56

2.06

12.66

0.29

24.00

0.25

3.93

0.10

1.87

1.86

13.97

0.28

26.78

0.28

平均含量±SD

Average content±SD

3.85±0.10

0.11±0.02

1.68±0.16

2.17±0.38

13.67±0.90

0.29±0.002

25.74±1.52

0.27±0.01

成分Component

不同吸收剂量下的显著性概率值（n=3）

Probability value of significance under difference absorbed dose

3 kGy

6 kGy

10 kGy

15 kGy

绿原酸Chlorogenic acid

0.305

0.690

0.014（＜0.05）

0.529

川芎嗪Ligustrazine

0.812

0.080

0.172

0.481

阿魏酸Ferulic acid

0.053

0.309

0.517

0.929

阿魏酸松柏酯Coniferly ferulate

0.259

0.478

0.031（＜0.05）

0.714

洋川芎内酯Senkyunolide

0.629

0.655

0.047（＜0.05）

0.965

正丁基苯酚N-butyl phenol

0.290

0.079

0.892

0.024（＜0.05）

藁本内酯Ligustilide

0.853

0.742

0.035（＜0.05）

0.819

欧当归内酯Levistilide

1.000

0.191

1.000

0.062

Yalu QIU, Peng GAO, Jiang HE, Qian CHEN, Yan WANG. Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of ⁶⁰Co-γ irradiation on its active components[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(5): 050303

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