A method on particles holograms analysis was proposed based on Hough transform and linear chirp transform. Particles sizes were calculated using the position of minimum point in space domain and frequency domain. After pretreatment of noise reduction filtering and edge detection to the holograms, string midpoint Hough transform was used to detect the center position of the particles, and the intensity distribution curve was described according to the cumulative normalized gray value of each circumference. Light intensity signal is applied to Fourier transform, combined with linear chirp transform for local spectral amplification, and then the accurate position of first minimum point in frequency domain can be known. Finally the particle sizes can be resolved by the obtained position in space and frequency domain. In terms of further particle spacing of particle holograms, numerical simulation results show that the method error were 2.5% or less.