A new Eu(III) complex {［Eu2(3,5-pdc)(3,4-pdc)2(H2O)4］·3H2O}n was synthesized by reacting 3,5-pyridinedioic acid (3,5-H2pdc) and 3,4-pyridinedioic acid (3,4-H2pdc) with Eu(ClO4)3 water solution at 170 ℃ by hydrothermal method. X-ray single crystal diffraction shows that the complex is a two-dimensional structure. The complex crystallized in monoclinic crystal system and P21/m space group. Its crystal cell parameters are a=0.598 20(4) nm, b=2.639 21(19) nm, c=1.147 58(9) nm, α=γ=90°, β=102.628 0(10)°. The complex was characterized by elemental analysis, infrared spectroscopy. The excitation and emission spectrum, fluorescence lifetime and luminescence quantum yield of the complex were tested. The results indicate that the complex can emit characteristic spectra of Eu (III). The fluorescence lifetime of 5D0→7F2 transition is 338 μs, and the absolute luminescence quantum yield is 24% under the excitation of 313 nm ultraviolet light.