Bypassing the limit in volumetric imaging of mesoscale specimens

Chaohao Chen; Zhen Liu; Dayong Jin

doi:10.1117/1.AP.1.2.020502

Journals >Advanced Photonics >Volume 1 >Issue 2 >Page 020502 > Article

Advanced Photonics
Vol. 1, Issue 2, 020502 (2019)

Bypassing the limit in volumetric imaging of mesoscale specimens

Chaohao Chen¹, Zhen Liu², and Dayong Jin^1、*

Author Affiliations

¹University of Technology Sydney, Institute for Biomedical Materials and Devices, Faculty of Science, Ultimo, New South Wales, Australia

²Southern University of Science and Technology, Department of Biomedical Engineering, Shenzhen, China

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DOI: 10.1117/1.AP.1.2.020502 Cite this Article Set citation alerts

Chaohao Chen, Zhen Liu, Dayong Jin. Bypassing the limit in volumetric imaging of mesoscale specimens[J]. Advanced Photonics, 2019, 1(2): 020502 Copy Citation Text

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The principle of subvoxel light-sheet microscopy using wide-range light-sheet illumination and large-view imaging. An off-axial and continuous scanning method is used to achieve fast acquisition of a series of large field-of-view images at low resolution (step I). Through an iterative optimization computational procedure, a voxel super-resolved image can be reconstructed (step II). To compensate the image deformation, voxel realignment is applied (step III).

Fig. 1. The principle of subvoxel light-sheet microscopy using wide-range light-sheet illumination and large-view imaging. An off-axial and continuous scanning method is used to achieve fast acquisition of a series of large field-of-view images at low resolution (step I). Through an iterative optimization computational procedure, a voxel super-resolved image can be reconstructed (step II). To compensate the image deformation, voxel realignment is applied (step III).

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