Cryogen spray cooling (CSC) in conjunction with laser therapy has been the clinical standard for hypervascular lesions. In order to optimize nozzle and enhance the cooling efficiency to improve the treatment of laser surgery, an experimental system of transient cryogen spray cooling is built and eight straight-tube nozzles with different diameter and length are designed. A thin film thermocouple is directly deposited on the cooling surface to measure the surface temperature during the cryogen spray cooling. An analytical expression based on Fourier′s law and Duhamel′s theorem is used to calculate surface heat flux from the temperature measurements. Based on the measurements and calculations, the effect of the eight straight-tube nozzles on the heat transfer dynamics of the cooling surface and the atomization characterics are comparatively studied. Additionally, the criterion to evaluate the cooling efficiency of different nozzles is proposed, and the variation of heat extraction from the cooling surface with different spray distances by different nozzles is given.