The microstructure features of welded joints after laser tailor-welding and hot stamping of Usibor 1500 steels are investigated. The mechanical properties of welded joints after hot stamping are tested by the room temperature tensile test. The results show that the microstructure of the weld seam is composed of coarse lath martensite and the unevenly distributed second phase with white color under the optical microscope. The white phase number decreases with the increase of coating removal parts. The tensile fracture pattern of the welded joint is related to the distribution of white phase in the weld seam. The removal state of coating has a significant effect on the performance of laser tailor-welded blanks. The removal of upper coating can significantly improve the performance of welded joints. The tensile property of welded joints is significantly improved with the number of the lower coating removal parts increases. The tensile strength of welded joint reaches the maximum value when all of the coatings are removed.