Fei Hu, Yanfei Liu, Xichen Li, Minghang Cao, Niancai Peng, Zhenxi Zhang. Convenient Nucleic Acid Detection Method and Point-of-Care Detection Device Based on CRISPR/Cas12a Molecular Diagnosis[J]. Chinese Journal of Lasers, 2022, 49(15): 1507402
- Chinese Journal of Lasers
- Vol. 49, Issue 15, 1507402 (2022)
Fig. 1. Flow chart of a convenient nucleic acid detection method based on CRISPR/Cas12a
Fig. 2. Schematics of instrument structure. (a) Physical map of experimental instrument; (b) schematic of overall structure of testing instrument; (c) schematic of optical cassette structure; (d) schematic of instrument base structure
Fig. 3. Optical inspection in minimalist mode. (a) Workflow chart of nucleic acid detection APP; (b) real-time fluorescent CRISPR reaction time; (c) positive result showed by nucleic acid detection APP; (d) negative result showed by nucleic acid detection APP
Fig. 4. Schematics of optical circuit. (a) Schematic of fluorescence recognition circuit for small portable device; (b) schematic of coaxial optical fiber detection principle
Fig. 5. Experimental detection limit in minimalist mode. (a) Results showed by nucleic acid detection APP; (b) fluorescence data analysis diagram
Fig. 6. Specific analysis (data represented as the mean ± standard deviation of three experimental replicates). (a) Specific analysis of different virus; (b) base mismatch specificity analysis
Fig. 7. Detection results comparison between our method and PCR method ( negative reaction time is replaced by 0 min)
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Table 1. Sequence information of DNA oligonucleotides and crRNA
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Table 2. Sequence information of nucleic acid mismatch
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