Biological ultra-weak luminescence is an electromagnetic signal from cells, which plays an important role in revealing the mechanism of electromagnetic biological effects. In order to study the biological effects and mechanism of extremely low frequency pulsed electric field, 1 Hz extremely low frequency high-voltage pulsed electric field based on the potential fluctuation frequency of corn cells was used to treat the corns during germination. The results showed that the germination process of corns was accelerated obviously and both the shoot length and root length of germinating corns were significantly longer than the control. Through the measurement and analysis of spontaneous luminescence and delayed luminescence on corns during germination, it was found that the spontaneous luminescence and delayed luminescence integral intensity of germinating corn were significantly increased under the action of 1 Hz extremely low frequency high-voltage pulsed electric field, which indicated that this specific pulsed electric field promoted the DNA synthesis and cell metabolism of the corns during germination. The coupled resonance of pulsed electric field and cell electric field in corn seeds may be the cause of biological effects of the extremely low frequency high-voltage pulsed electric field.