Two dimensional optical trapping of gold nanorods suspended in aqueous solution was investigated by using a single focused laser beam and the interaction between two gold nanorods was studied by measuring the scattering of gold nanorods trapped in the optical trap with a dark-field microscopy.It was found that the two gold nanorods simultaneously trapped would keep certain distance due to the repulsive interaction, which suppressed the localized surface plasmonic coupling between the two gold nanorods.The experimental results will provide technological and experimental references for the optical trapping of gold nanorods and their application in the probing of biomolecules.