A novel ultraviolet absorption spectrometry method was developed for the quantitative determination of HABS by adding β-cyclodextrin with the molar ratio of 1∶1 in strong interference aqueous solution. The results indicated that the effect of several common interfering flooding agents (SAS, OP-10, HPAM) on the determination of HABS could be greatly reduced in β-cyclodextrin aqueous solution. Thus, the determination errors of the determined HABS were less than 2.0% under strong interference, and the detection limit (S/N=3) of the method could be also as high was 8.3～9.1 10－4 mg·L－1. Various characterization results including 1H-NMR, TG-DSC and FTIR showed the interaction between β-cyclodextrin and HABS. The results of 1H-NMR analysis showed that HABS molecule could enter into the interior of the cavity of β-cyclodextrin molecule. TG-DSC analysis exhibited that the stable inclusion of β-cyclodextrin and HABS could be automatically formed. The interactions between the functional groups of β-cyclodextrin and HABS were showed by FTIR analysis, which also exhibited that the stable inclusion could be formed by HABS entering from the narrow or the broad mouth of the β-cyclodextrin. The interference of the UV spectrum of HABS could be reduced by β-cyclodextrin since the interaction between β-cyclodextrin due to the interaction between β-cyclodextrin and HABS in the inclusion complex.