The attacking orbits of interception satellites were explored when target satellites flied with several small satellites in concomitant formation in anti-satellite combination. By taking an interception satellite with a low continuous thrust to transfer orbit for a researching object, an attacking orbit optimization method based on genetic algorithm was proposed. A dynamic defending model of the small formation satellite was taken as environment model, and comprehensive fitness function was constructed according to the requirements of orbit safety and fuel saving. Then, the coding method, selection operator, crossover operator and the mutation operator were designed for proposed algorithm. A simulation experiment was performed on the MATLAB platform. The simulation results show that the interception satellite hits the target satellite at 650 s, spending 410 s. The algorithm proposed searches an optimal attacking path and has a good convergence and stability. As compared with the same kind of optimization method, this algorithm effectively reduces the boot time of rockets, and reduces the task loads of orbit maneuver attitude control of the interception satellite.