Graphene is doped into the photoresist which is made by mixing the 2-benzyl-2-(dimethylamino)-4′-morpholino-butyroph and pentaerythritol triacrylate. A series of 2D image and 3D microstructure were fabricated based on the technology of femtosecond laser two-photon polymerization(TPP). The infiltration of the mixture was characterized by the hydrophilic angle test, and the penetration of the mixture was characterized by laser penetration depth test. The experimental results show that the mixture with graphene still has strong binding force with glass and laser penetration. At last, 2D image and 3D microstructure were characterized by Raman mapping and scanning electron microscope (SEM). It is confirmed that graphene can be relatively evenly mixed in microstructure by using deionized water as dispersion liquid, and the composite microstructure is more stable and the appearance is more uniform than the pure photoresist microstructure.