The electro-optical characteristics of negative VA-LCD driven by different voltage are measured by UV-Vis8500 double beam ultraviolet (UV)/ visible spectrophotometer. The threshold characteristics of three primary colors and the characteristics variation with wavelength are analyzed. The results show that threshold voltage (Vth), saturation voltage (Vsat) and mid-value voltage (V50) all increase gradually with the increasing of wavelength. And the difference between Vsat and Vth becomes larger gradually with the increasing of wavelength. Common used mean voltage V50 of three kinds of central wavelength such as 500.0 nm, 550.0 nm and 600.0 nm is taken as driving voltage to analyze. The results can provide certain guidance for choosing the driving voltage of colorful liquid crystal display (CLCD).