This paper mainly discussed the optimum conditions of hydride generation atomic fluorescence spectrometry (HG-AFS) for the determination of selenium content in eggs, then the analytical method for the determination of selenium content by mixed acid digestion hydride generation atomic fluorescence spectrometry was established, and through testing the selenium content of ordinary eggs, native eggs and dark eggs, we hoped to provide a theoretical and practical reference for people to choose eggs. In order to improve the precision and accuracy of the method to detect the selenium content in eggs by atomic fluorescence spectrometry, the mixed acid ratio, prereducing agent concentration and KBH4 concentration were compared and analyzed, and the feasibility of test result was detected by calculating the precision, recovery rate and the minimum detection limit. The results showed that the samples were dissolved overnight after the mixture of concentrated nitric acid and perchloric acid with a volume ratio of 1∶1, then heated to clear on the micro control digital display electric heating plate at 200 degrees, then the temperature of the electric heating plate was adjusted to 160 degrees, after the conical bottle temperature was cooled to room temperature, 5 mL of 6 mol·L-1 HCl was added. The conical bottle was heated on the electric heating plate again, and removed after the solution was clear. After cooled to the room temperature, the conical bottle solution was transferred to 100 mL capacity bottle, and 1 mL 10% potassium ferricyanide solution was added, and 10% hydrochloric acid was used to shake and be measured at the same time, and the sample blank control was made at the same time. The analytes were detected by a high-performance hollow cathode selenium lamp with a deoxidizer of 1.5% KBH4 solution and the carrier fluid of 2% HCl. At the same time, the different reasons of selenium content between varieties of eggs were discussed. Under the optimum conditions and the working state, the selenium contents showed a good linear relationship in the concentration range from 0 to 8 g·L-1, and the standard curve equation of selenium is IF=114.19C＋1.30, and the correlation coefficient of the standard curve was 0.999 9, and the minimum detection limit was 0.01 μg·L-1, and the relative standard deviation was 0.07%~0.72%, and added standard recovery was 96.12%~99.1%. The optimum conditions of hydride generation atomic fluorescence spectrometry (HG-AFS) for the determination of selenium content in eggs were established, and the method is simple and easy to operate, with high precision and sensitivity. Using the method to test selenium content of ordinary eggs, native eggs and dark eggs, the results showed that the selenium content of dark eggs, native eggs and ordinary eggs were 0.191, 0.195, 0.141 mg·kg-1, respectively. The selenium content of dark eggs was not significant than that of native eggs (p>0.05), but the selenium content of dark eggs and native eggs were significantly higher than that of ordinary eggs (p<0.05). This study provides a theoretical basis for the scientific detection of selenium content in eggs and people’s choice.