The Fe901 alloy is coated on a 45 steel surface by laser cladding. The microstructure, phase compositions, and micro-hardness of the laser cladding coating are tested. The wear experiment of the laser cladding coating and the 45 steel samples are conducted using the dry sliding method. The results show that the laser cladding coating has a uniform and compact microstructure, and the phase compositions are primarily composed of martensitic phase and several inter-metallic compounds such as CrFeB and Cr₇C₃. The laser cladding coating shows an average micro-hardness of 718 HV, which is considerably higher than that of the substrate (269 HV). Abrasive wear, fatigue spalling, and oxidative wear are the main wear mechanisms of 45 steel. The wear mechanism of the laser cladding coating is mainly abrasive wear. Under the dry sliding condition and with the loads of 10, 20, and 30 N, the friction coefficient of the laser cladding coating is smaller than that of the 45 steel, and the relative wear resistance is 4, 18, and 20 times that of the 45 steel, respectively. Laser cladding of Fe901 alloys can considerably improve the wear resistance of 45 steel.