The present study investigated the possibility of using near-infrared reflectance spectroscopy (NIRS) for quantitative determination of oligosaccharides, including sucrose, raffinose and stachyose in soybean products. One hundred and sixty samples (de-hulled soybean meal, extruded soybean meal, extruded full-fat soybean and fermented soybean meal) were collected and screened, and furthermore forty artificial soybean meal samples were prepared. Calibrations were performed by partial least squared algorithm with different derivatives, multiplicative scatter correction or normalization vector spectral pretreatment. The coefficients of determination for calibration (R2cal), coefficients of determination for cross validation (R2cv) and RPDcv in models were 0.99, 0.95, 0.98; 0.98, 0.94, 0.97; 7.24, 4.13, 5.98; respectively. The results supposed that NIRS can be a quantitative determination method of oligosaccharides in soybean products except fermented soybean meal.