A new temperature calibration equipment is designed based on the thermoelectric cooler module, and a novel temperature calibration method using dynamic multi-section optical fiber is applied and its feasibilty and accuracy are verified by experiments. With the changing of external temperature, the temperature at any point along the optical fiber can be sensed and demodulated accurately. This temperature demodulation method is accurater, more stable and suitable for the applications under the complex practical environment. The error of the multistage temperature calibration is less than 1 ℃.