The seedling of wheat (jinmai 8) was exposed to He-Ne laser (5 mW·mm-2), enhanced UV-B radiation (10.08 kJ·m-2·d-1) and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation.The research discovers, light absorbability of thylakoid membrane of wheat seedling leaves is decreased by enhanced UV-B radiation, Mg2+-ATPase and Ca2+-ATPase activity are also depressed. Activity of photophosphorylation(PSP), cyclic PSP and non-cyclic PSP, is restrained by enhanced UV-B radiation.The enhanced UV-B radiation causes damage on thylakoid membrane of wheat seedling. But certain amounts of He-Ne laser can partially repair UV-B damage on thylakoid membrane wheat seedling leaves, and reconstruct light absorbability of thylakoid membrane of wheat seedling. Mg2+-ATPase and Ca2+-ATPase activity are also raised. Cyclic PSP and non-cyclic PSP activity is increased by He-Ne laser.The SDS-PAGE result shows that different treatment groups can not induce obvious differences on the polypeptide compositions of thylakoid membrane, which will be reduced by enhanced UV-B radiation,but increased after He-Ne laser radiation.