A method that uses radio frequency (RF) spectroscopy to evaluate the alignment of an optical lattice is proposed and demonstrated. A one-dimensional (1D) optical lattice is applied along the long axis of a cigar-shaped Bose-Einstein condensate (BEC) in a magnetic trap. The RF spectra of condensates with and without the optical lattice are analyzed, measured, and compared with the condition in which the lattice is misaligned with the BEC. The proposed method greatly optimizes the optical alignments of the lattices.