The intensity tuning profile is the base for mode selection and frequency stabilization in ring laser gyro (RLG), which is decided directly by the gaintoloss ratio (GTLR). To improve the performance of the RLG, the dependence of the output bandwidth on the GTLR for twomode RLG with the gain medium making of the mixture of Neon isotopes (Ne20 and Ne22) is numerically analyzed, with the consideration of comprehensive broadening (both homogeneous broadening and inhomogeneous broadening). It is shown that the output bandwidth increases monotonically with the GTLR of RLG. The intensity tuning profiles with different GTLRs of RLG are simulated and the results show that the intensity tuning profiles can be divided into three typical forms, according to the relative value of the output bandwidth to the adjacent longitudinal mode spacing, exhibiting great agreement with the experimental ones. The potential influence of improper GTLR selection on the application performance of RLG is analyzed and experimentally demonstrated. The ideal selection of GTLR for RLG is proposed, that is, the output bandwidth of the RLG should be approximately equal to its adjacent longitudinal mode spacing.