To meet the relative radiometric correction requirements of optical remote sensor with large aperture and wide viewing field, the uniformity of integrating spheres light source was studied. Base on sphere cavity radiant theory, the output uniformity of integrating spheres reference light source was analyzed from radiant emittance distribution and mounting position of emitting module. The point source(tungsten-halogen lamps) and Lambert radiant type of surface light source(LEDs) are respectively mounted on the position within ±30°~±36.8° and ±30°~±45° to the exit normal of integrating spheres through optimal design. Using uniformity analysis result, integrating sphere with diameter of 3 m and diameter exit port of 1 m was designed based on LEDs and tungsten-halogen lamps emitting modules, and the angular and planar uniformity were measured. The results show that the planner uniformity within Φ890 mm and the angular uniformity within ±60°are better than 99% to the exit normal.