The relationship between optical property parameters and the change of hyperosmotic agents were evaluated. Porcine skins, medicated without and with biocompatible hyperosmotic agents-dimethyl sulfoxide and thiazone, were measured by FT-IR spectrophotometer and double-integrating-spheres system. Optical property parameters of samples were calculated by inverse adding-doubling modeling method. The results demonstrated that with the course of the penetration of biocompatible hyperosmotic agents into porcine skin tissue, optical property parameters(absorption coefficient and scattering coefficient)of tissue samples have got decrease obviously along with the depth of light penetrating into tissue increased. In conclusion, the optical property parameters of bio-tissues could be changed by use of both agents, furthermore, thiazone has led to the better clearing than dimethyl sulfoxide.