In this paper, a double-integrating-spheres system, basic principle of measuring technology of ray radiation, optical model of biological tissues were used to study the optical properties of human tissues. The results of measurement showed that the parameters of optical properties of human normal bladder and human bladder cancer tissue at 808 nm laser and the linearly polarized laser in Kubelka-Munk two-flux model had prominent distinction (P<0.01). The parameters of optical properties of human normal bladder tissue for 808 nm laser and the linearly polarized laser had all distinction. Absorption coefficient and effective attenuation coefficient among all parameters of optical properties of human normal bladder tissue had obviously distinction. All parameters of optical properties of human bladder cancer tissue for 808 nm laser and the linearly polarized laser had not prominent distinction (P>0.05) which showed that the depolarization of humanbladder cancer tissue to 808 nm linearly polarized irradiation was very big. The parameters of optical properties of humannormal small intestine tissue at 808 nm lase rand the linearly polarized laser irradiation had all prominent distinction (P<0.01), which showed that the depolarization of human normal small intestine tissue to 808 nm linearly polarized irradiation was smaller. Distribution of light intensity of three kinds of human tissues at 808 nm laser and the linearly polarized laser irradiation in Kubelka-Munk two-flux model had obviously dissimilitude.