Sea surface temperature is of great significance in the ocean research. One way to obtain SST is through the retrieval of remote sensing data. How to construct EOS/MODIS data for retrieval of the SST based on the C# and IDL is described, the function of the software is discussed, and the data process and details of the retrieval algorithms are given. Finally, the South China Sea is taken as an example to compare the retrieval results with the observation data. The result shows that the linear correlation coefficient between the inversion results and the measured data is 0.91, and the standard error is 0.7℃. The inversion results are expected to provide reference for marine exploration.