An infrared seeker refrigeration gas supply test system is used to determine the cooling time of an infrared seeker in a weapon system to verify the cooling effect of high-pressure. This study introduces the composition and structural design of the system by primarily using high-pressure argon as the refrigeration gas source. Argon controls and simulates the actual working temperature of the seeker through a high-and low-temperature test system, thereby controlling the cooling time, pressure, temperature test, and imaging display through the data acquisition system. To overcome common problems of systems in engineering applications, structural improvement schemes are proposed. Through several years of production and application, it is verified that the system can meet the requirements of batch testing for infrared seekers in weapon systems.