Fig. 1. (a) Schematic of the LIHM system setup; (b) photograph of the system.

Fig. 2. Flowchart of the reconstruction process in our method.

Fig. 3. Numerical simulation to demonstrate our method with a two-layer sample. (a) The top layer of the sample; (b) the bottom layer of the sample; (c) one of the in-line holograms of the two-layer sample; (d) the segmented hologram of the USAF target elements obtained from (c); (e) the holographic reconstruction result according to (c); (f) PSR reconstruction using multiple low-resolution images of (e); (g) PSR reconstruction using multiple segmented low-resolution images obtained from (d).

Fig. 4. Experiment with a two-layer sample, where the top layer is a USAF target, and the bottom layer contains aggregations of microspheres. (a) The schematic of the two-layer sample; (b) one of the in-line holograms of the two-layer sample; (c) the conventional holographic reconstruction result according to (b); (d) the enlarged image of the region indicated in (c); (e) PSR reconstruction using multiple low-resolution images of (d); (f) PSR reconstruction using multiple low-resolution images acquired by segmentation and reconstruction of the enlarged region shown in (d).

Fig. 5. Experiments with the sample of water solution containing the algae Euglena gracilis. (a) One of the in-line holograms; (b) the conventional holographic reconstruction according to (a), where the target alga and some adjacent objects can be observed; (c) the segmented in-line hologram of the target alga as indicated in (a); (d) the enlarged image of the target alga as indicated in (b); (e) PSR reconstruction of the target alga without using the segmentation technique; (f) PSR reconstruction of the target alga with applying the segmentation technique; (g) microscope image of similar alga observed under a 20× objective.

Fig. 6. Atlas of algae images obtained by PSR reconstruction after the segmentation technique in our LIHM system.