• Experiment Science and Technology
  • Vol. 21, Issue 6, 122 (2023)
Yunfan QUAN1, Yingfei ZENG1, Jinqian LI1, Tingting FU1, Qinghui SUN1, Hao XIU1, and Xiuji CUI2、*
Author Affiliations
  • 1School of Tropical Medicine, Hainan Medical University, Haikou 571199, China
  • 2School of Basic Medical Sciences and Life Sciences, Hainan Medical University, Haikou 571199, China
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    DOI: 10.12179/1672-4550.20230054 Cite this Article
    Yunfan QUAN, Yingfei ZENG, Jinqian LI, Tingting FU, Qinghui SUN, Hao XIU, Xiuji CUI. Evaluation of the Accuracy and Stability of Oxidase Test in Microbiology Experimental Teaching Course[J]. Experiment Science and Technology, 2023, 21(6): 122 Copy Citation Text show less

    Abstract

    The study aims to increase the accuracy and stability of the oxidase test due to the frequent false-positive and false-negative to the oxidase test during the microbiology experimental teaching. Here, we optimized experimental procedure and tested various culture mediums for pseudomonas aeruginosa by evaluating various bacterial culture medium and oxidase test kit to find a proper oxidase test protocol. Colonies were picked up with a sterilized toothpick, glass stick, or directly picked up with the paper disk. As a result, pseudomonas aeruginosa cultured in a slant agar medium frequently showed false positivity in the oxidase test, whereas pseudomonas aeruginosa cultured in regular agar plate or blood agar plate didn’t show any false positive. The experiment results showed that the blood agar was the best medium for bacterial culture for the oxidase test, and the oxidase test paper disk or N,N-Dimethyl-p-phenylenediamine dihydrochloride test paper showed a similar result in the oxidase test.
    Yunfan QUAN, Yingfei ZENG, Jinqian LI, Tingting FU, Qinghui SUN, Hao XIU, Xiuji CUI. Evaluation of the Accuracy and Stability of Oxidase Test in Microbiology Experimental Teaching Course[J]. Experiment Science and Technology, 2023, 21(6): 122
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