Hyperspectral technology is the base for CO retrieval with high accuracy. With the characteristics of hyperspectral technology and the requirement of accuracy of atmospheric column CO retrieval, after absorption features analysis of atmospheric CO, an absorption band appropriate for research is selected. On the basis of retrieval principle and retrieval method, effects of spectral resolution, terrain height, initial value of CO column and surface albedo on retrieval accuracy are investigated using forward model LBLRTM. The result demonstrates that in the process of CO retrieval thoughtful considerations are desired for sensitive factors above-mentioned so as to improve the accuracy.