The removal of a transition film from an ion-proof microchannel plate affects the performance and quality of the latter. In this paper, the removal technology of nitrocellulose transfer membrane is studied theoretically and experimentally. A nitrocellulose transition film was removed by hydrogen reduction heat treatment and ultraviolet irradiation. The surface composition and properties of the nitrocellulose were measured and analyzed by X-ray photoelectron spectroscopy, plate resistance, and current gain test. The removal of nitrocellulose and the performance of the microchannel are discussed. It is discovered that both hydrogen reduction heat treatment and ultraviolet irradiation can effectively remove the nitrocellulose membrane and sustain the stable performance of the microchannel plate.