The correlation between the Raman stretching frequency shifts of a ethyl group and the concentration of the ethanol in aqueous solution was studied, and the new methods to determine the ethanol content in aqueous solution were obtained. The Raman spectra in the range of 500~4 000 cm-1 were obtained on the Renishaw 1000 Raman spectrometer in the room temperature,which shows that the shifts of C-H stretching vibration bands give a negative correlation with the ethanol concentration. Furthermore, the simple linear regression was employed to analyze the relation between the shift of band position and the concentration, and then a futher research using Multiple Linear Regression(MLR) was performed to obtain a function between the ethanol concentration and the shifts of bands on the base of this. The results show that for both the C-H symmetric stretching vibration band and the -CH3 asymmetric stretching vibration band, there are significant linear relations between their parameters. In addition, the determination coefficient of the MLR model has reached 0.98, which shows a perfect linear relation. These results indicate that the ethanol concentration in the aqueous solution can be measured by the Raman stretching frequency shifts of ethyl group.