Jiayu YANG, Juntian ZENG, Bangchao XI, Guoqiang LIU, Shaolei HUANG, Dongxu ZHANG. Multiple real-time fluorescence detection system for rapid on-site nucleic acid detection[J]. Journal of Applied Optics, 2023, 44(4): 859
- Journal of Applied Optics
- Vol. 44, Issue 4, 859 (2023)
Fig. 1. Excitation optical path and emission optical path
Fig. 2. Semi-divergence angle test device
Fig. 3. Comparison diagram of emission optical path simulation and illumination
Fig. 4. Multi-channel rotation integrated module
Fig. 5. Embedded structure between module brackets
Fig. 6. Physical image of proposed fluorescence detection system
Fig. 7. Test results of fluorescence dye gradient for each channel
Fig. 8. Test results of fluorescence crosstalk between channels
Fig. 9. Test results of sample amplification detection
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Table 1. Semi-divergence angle measured at each end of optical path（°）
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Table 2. Distance between elements of emission optical pathmm
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Table 3. Dilution concentration of fluorescent dyesμg·mL−1
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Table 4. Detection limit test results of fluorescent dyes
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Table 5. Gradient test results of fluorescent dyes
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Table 6. Repeatability test results of fluorescence intensity
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Table 7. Fluorescence crosstalk test results based on HEX fluorescent dyespA
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Table 8. Fluorescence crosstalk test results based on CY5 fluorescent dyespA
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