Photon-counting detection is a key technique for detection of weak intensity light and high-speed imaging study. A photon-counting detection and imaging experimental setup was designed and built. Its structure and working principle were introduced in detail. The experimental hardware was composed of Avalanche Photodiode (APD) arrays, suitable driving and quenching circuits and NI-PCI high speed data acquisition card. System software and data acquisition display interface were programmed with Labview and VC++ combination. Weak photon irradiated on the avalanche photodiode which works on Geiger mode was collected by high speed data collection card continuously and processed with personal computer software. Finally, the photo-counting pulse image was acquired by this system. The experimental setup provides a powerful tool for very faint luminescence detecting and high-speed imaging study.