This work synthesizes a large class of multifunctional CdTe@Fe3O4/P(NIPAM-co-AA) composite microspheres with superparamagnetic and fluorescent properties by using the polymer template method. The P(NIPAM-co-AA) microspores are obtained by inverse suspension emulsion polymerization. Magnetic nanoparticles were doped into the networks of the polymer template microspheres by a simple swelling method. TEOS and APTES were chosen to achieve amino- functionalization of the magnetic microsphere surface. Furthermore, mercaptoacetic acid modified- CdTe quantum dots reacted with amino functional microsphere, which finally resulted in the mono-dispersed magnetic fluorescent polymeric microspheres. Several techniques, e.g. scanning electron microscope, transmission electron microscopy, fluorescence microscopy and fluorescence spectrophotometer, etc. were used to examine structures and properties of the resulting composites. In summary, the resulting composite microspheres are as large as about 30 μm and exhibit excellent superparamagnetism and high efficient fluorescence quantum yield. The saturation magnetization reaches 5.4 emu/g. This approach to generating multifunctional composite microspheres by combining the magnetism and fluorescence onto micrometer copolymers could not only avoid difficulties in separation of nano-particles, but also lay bases for many potential applications in various fields.