• PhotoniX
  • Vol. 4, Issue 1, 6 (2023)
Chang Liu1、2、3、†,*, Wilhelm Eschen1、2、3、†, Lars Loetgering1、2、3、4, Daniel S. Penagos Molina1、2、3, Robert Klas1、2、3, Alexander Iliou5, Michael Steinert1, Sebastian Herkersdorf6, Alexander Kirsche1、2、3, Thomas Pertsch1、7, Falk Hillmann5、8, Jens Limpert1、2、3、7, and Jan Rothhardt1、2、3、7
Author Affiliations
  • 1Institute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University Jena, Albert- Einstein-Straße 15, 07745 Jena, Germany
  • 2Helmholtz-Institute Jena, Fröbelstieg 3, 07743 Jena, Germany
  • 3GSI Helmholtzzentrum für Schwerionenforschung, Planckstraße 1, 64291 Darmstadt, Germany
  • 4Present address: Carl Zeiss AG, Carl-Zeiss-Promenade 10, 07745 Jena, Germany
  • 5Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (Leibniz- HKI), Adolf-Reichwein-Str. 23, 07745 Jena, Germany
  • 6Department of Pharmaceutical Microbiology, Hans Knöll Institute (HKI), Friedrich-Schiller- University Jena, Winzerlaer Straße 2, 07745 Jena, Germany
  • 7Fraunhofer Institute for Applied Optics and Precision Engineering, Albert-Einstein-Str. 7, 07745 Jena, Germany
  • 8Present address: Biochemistry/Biotechnology, Faculty of Engineering, Hochschule Wismar University of Applied Sciences Technology, Business and Design, Philipp-Müller-Str. 14, 23966 Wismar, Germany
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    DOI: 10.1186/s43074-023-00084-6 Cite this Article
    Chang Liu, Wilhelm Eschen, Lars Loetgering, Daniel S. Penagos Molina, Robert Klas, Alexander Iliou, Michael Steinert, Sebastian Herkersdorf, Alexander Kirsche, Thomas Pertsch, Falk Hillmann, Jens Limpert, Jan Rothhardt. Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging[J]. PhotoniX, 2023, 4(1): 6 Copy Citation Text show less

    Abstract

    Table-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli) cells at 13.5 nm wavelength. We find that the EUV spectral region, which to date has not received much attention for biological imaging, offers sufficient penetration depths for the identification of intracellular features. By implementing a position-correlated ptychography approach, we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest. The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens. Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science.
    Chang Liu, Wilhelm Eschen, Lars Loetgering, Daniel S. Penagos Molina, Robert Klas, Alexander Iliou, Michael Steinert, Sebastian Herkersdorf, Alexander Kirsche, Thomas Pertsch, Falk Hillmann, Jens Limpert, Jan Rothhardt. Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging[J]. PhotoniX, 2023, 4(1): 6
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