Amine polyether for construction of high performance drilling fluid is a polyether terminated by a primary or secondary amine group. The amine group is usually a primary amine group, secondary amine group or tertiary amine group containing active hydrogen. As a highly effective inhibitor, amine polyether has been used more and more in drilling fluids. However, there are few researches on its physicochemical properties, especially the determination of amine group content which plays a key role in inhibition. In this paper, a determination method for the total amine value of amine polyether used in drilling fluid is established through condition optimization, and the total amine value of amine polyether with different molecular weights and different functional degrees is determined, with the relative average deviation ≤ 3%. The establishment of this method has important guiding significance for the quality control and field applications of amine polyether.